skip navigation

Add your conference to our Justice Events calendar


Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Library collection. To conduct further searches of the collection, visit the NCJRS Abstracts Database. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

  NCJ Number: NCJ 236689   Add to Shopping cart   Find in a Library
  Title: Improving the Efficiency of DNA Casework Analysis through Simple, Effective, PCR-based Screening Methods
  Document URL: PDF 
  Author(s): Janice A. Nicklas Ph.D. ; Eric Buel Ph.D.
  Date Published: 2011
  Page Count: 116
  Annotation: The major aims of this project were to develop a fast, simple profiling method for sample screening that can select DNA samples that would be most probative, as well as to develop a test for determining a sample’s DNA degradation state.
  Abstract: Regarding the effort to develop a simple profiling method for selecting DNA samples that would be most probative, researchers succeeded in developing a technique that can discriminate between 95-99 percent of samples from different individuals. Multiplex SNP assays were developed by using a melting FRET technique. In this assay, two probes are present. These are a sensor with a perfect match to one allele (with fluorophore) but with one mismatch to the other allele, as well as an anchor probe (with quencher). As PCR proceeds, fluorescence is quenched; and in the melting phase, fluorescence is gained. Determination of which allele (s) is present depends on the melting temperature at which the fluorescence is regained. Several assays were developed for the six- color Corbett RG6000 and for other four-color real-time instruments. Researchers also added an additional part to the first aim of the project, i.e., the development of a new assay for fast determination of stain donor using high resolution melting (HRM) of STRs. HRM goes beyond classical melt curve analysis by studying the melt in much finer detail using special DNA dyes such as Eva Green. In accomplishing the second aim of the research, i.e., the development of a test for sample DNA degradation state, researchers designed a multiplex PCR with two overlapping Alu amplicons, using the Plexor technology. This report describes how this technology can be used to obtain and quantitative measure of DNA degradation state. 57 figures, 36 references, and a listing of publications and presentations in which the research findings are presented
  Main Term(s): Police policies and procedures
  Index Term(s): Testing and measurement ; Victim identification ; Suspect identification ; Efficiency ; Productivity ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; NIJ final report
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2005-DA-BX-K003
  Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
  Type: Program/Project Description ; Report (Study/Research)
  Country: United States of America
  Language: English
  To cite this abstract, use the following link:

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.