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NCJ Number: NCJ 236689   Add to Shopping cart   Find in a Library
Title: Improving the Efficiency of DNA Casework Analysis through Simple, Effective, PCR-based Screening Methods
Author(s): Janice A. Nicklas Ph.D. ; Eric Buel Ph.D.
Date Published: 2011
Page Count: 116
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2005-DA-BX-K003
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Program/Project Description ; Report (Study/Research)
Language: English
Country: United States of America
Annotation: The major aims of this project were to develop a fast, simple profiling method for sample screening that can select DNA samples that would be most probative, as well as to develop a test for determining a sample’s DNA degradation state.
Abstract: Regarding the effort to develop a simple profiling method for selecting DNA samples that would be most probative, researchers succeeded in developing a technique that can discriminate between 95-99 percent of samples from different individuals. Multiplex SNP assays were developed by using a melting FRET technique. In this assay, two probes are present. These are a sensor with a perfect match to one allele (with fluorophore) but with one mismatch to the other allele, as well as an anchor probe (with quencher). As PCR proceeds, fluorescence is quenched; and in the melting phase, fluorescence is gained. Determination of which allele (s) is present depends on the melting temperature at which the fluorescence is regained. Several assays were developed for the six- color Corbett RG6000 and for other four-color real-time instruments. Researchers also added an additional part to the first aim of the project, i.e., the development of a new assay for fast determination of stain donor using high resolution melting (HRM) of STRs. HRM goes beyond classical melt curve analysis by studying the melt in much finer detail using special DNA dyes such as Eva Green. In accomplishing the second aim of the research, i.e., the development of a test for sample DNA degradation state, researchers designed a multiplex PCR with two overlapping Alu amplicons, using the Plexor technology. This report describes how this technology can be used to obtain and quantitative measure of DNA degradation state. 57 figures, 36 references, and a listing of publications and presentations in which the research findings are presented
Main Term(s): Police policies and procedures
Index Term(s): Testing and measurement ; Victim identification ; Suspect identification ; Efficiency ; Productivity ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; NIJ final report
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=258704

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