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NCJ Number: NCJ 237840     Find in a Library
Title: Tools for Improving the Quality of Aged, Degraded, Damaged, or Otherwise Compromised DNA Evidence
Author(s): John R. Battista
Date Published: 02/2012
Page Count: 30
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2007-DN-BX-K146
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Document: PDF 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: This study’s primary objective was to develop tools that will facilitate STR DNA genotyping by improving the quality of the DNA found in degraded forensic samples and enhancing the ability to retrieve amplifiable DNA from forensic samples.
Abstract: The study succeeded in developing an in-vitro method that uses three purified proteins in enabling the selective capture of specific DNA sequences. This method relies on the use of a targeting DNA fragment, a sequence that is complementary to the sequence the examiner wishes to retrieve. Combining this fragment, the three proteins, and appropriate cofactors allows recovery of homologous DNA from solution with high efficiency. This process, referred to as in-vitro double-strand break repair reaction, requires the following three proteins: RecA protein, single-strand binding (SSB) protein, and a DNA polymerase. To date, all three proteins are obtained from Escherichia coli. RecA and SSB are needed for the identification of the region of homology and promoting invasion by the 3’ end of single strand DNA molecule recombining with duplex DNA. The DNA polymerase extends the 3’ ends, sealing the spliced fragments formed by the action of RecA and SSB. This reaction can be used to repair double strand breaks found between the STR and the primer sites for STR amplification. If the targeting fragment includes at its 5’ end a feature that allows efficient retrieval such as biotin that can be harvested with streptavidin-coated magnetic beads, recombined fragments can be isolated and concentrated for further manipulation. In order to establish that in-vitro double strand break repair is possible, an assay was developed that used the plasmid pUC19 cleaved by a pair of restriction enzymes. 6 figures, 2 tables, and 26 references
Main Term(s): Police policies and procedures
Index Term(s): Victim identification ; Suspect identification ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; NIJ final report
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=259872

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