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NCJ Number: NCJ 247082   Add to Shopping cart   Find in a Library
Title: Development of a DNA-Based Multi-Species Identification and Quantification Assay
  Document URL: PDF 
Author(s): K'Lynn Solt ; Sree Kanthaswamy
Date Published: 06/2014
Page Count: 59
  Annotation: Based on the research reported here, the authors published four papers on species-specific DNA quantification of human, dog, cat, livestock, and poultry.
Abstract: Species identification of forensic samples allows the analyst to determine the appropriate test when evaluating a wide range of biological evidence. Since most animal short tandem repeats (STR) analyses are species-specific, it is important to know the species of origin of the sample before attempting STR typing. The ability to accurately detect and quantify target DNA in mixed-species samples is crucial when target templates may be overwhelmed by non-target DNA. The use of accurate species-determining tests will enable analysts to optimize the typing tests used in a case and minimize the consumption of limited samples. This project combined the strengths of DNA-based taxon identification with that of a nuclear marker for DNA quantification into a simple and quick duplex single nucleotide polymorphism TaqMan assay. This robust and cost-efficient method for taxon differentiation and DNA quantification will be developed for test commercialization. The speciation and quantification assays have undergone scientific scrutiny, so as to ensure accuracy, precision, and reproducibility of the procedure; species-specificity, sensitivity, stability, and mixture analyses were part of its validation. Conditions for primers and probes were that these reagents identify sequences within target species that were conserved but contained sufficient polymorphisms such as SNPs that would enable the distinguishing of one target species from another. Target regions within these species’ genomes were found for species-specific primers and probes. 8 figures, 4 tables, and 60 references
Main Term(s): Criminology
Index Term(s): DNA fingerprinting ; DNA Typing ; Animal DNA ; DNA extraction ; Mixed DNA profiles
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2008-DN-BX-K288
Sale Source: NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
Type: Report (Study/Research) ; Research (Applied/Empirical) ; Report (Grant Sponsored)
Country: United States of America
Language: English
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