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NCJ Number: 253073 Find in a Library
Title: Evaluating the Efficiency of the Use of the Qiagen QIAsymphony with High Throughput Y-screening as an Alternative to Conventional Serology
Author(s): Jason Chute
Date Published: July 2019
Page Count: 12
Sponsoring Agency: Marshall University Research Corporation

National Institute of Justice (NIJ)
Washington, DC 20531
US Dept of Justice NIJ Pub
Washington, DC 20531
Grant Number: 2015-R2-CX-K039
Sale Source: US Dept of Justice NIJ Pub
810 Seventh Street, NW
Washington, DC 20531
United States of America
Document: PDF
Type: Program/Project Description; Report (Grant Sponsored); Report (Study/Research); Report (Summary); Research (Applied/Empirical)
Format: Document; Document (Online)
Language: English
Country: United States of America
Annotation: This is the Final Summary Overview of the findings, methodology, and criminal justice implications of a research project whose goal was to develop and evaluate an accurate high throughput screening method for the presence of male DNA (otherwise known as Y-screen) as an option for reducing forensic DNA laboratory backlogs of untested sexual assault kits (SAKs).
Abstract: The distinctive aspect of the method tested is taking a larger initial cutting from the evidence compared to traditional serology or other Y-screen methods and then extracting the cells from the sample with Phosphate Buffered Saline (PBS). This extraction lysate is referred to as the LCS or liquid cellular slurry. A set amount of the LCS is further extracted by using DTT-based extraction buffer on the Qiagen QIAsymphony that will lyse all cells present in the LCS. Quantitative PCR with simultaneous male and human DNA quantitation setup is then performed on the extracted LCS. Analysis or screening of samples based on the presence or absence of male DNA can then occur. If necessary, a differential separation and extraction can be performed on the LCS. The LCS should be a homogenous sampling prior to screening, which should create an accurate representation of the DNA on the evidence. This project compared and evaluated laboratory efficiency of the LCS method, This is the newly released SWGDAM recommended method and conventional serology. The study concluded that performing a Y-screen using the Qiagen QIAsymphony provides more accurate sampling, increased correlation to DNA STR results, and a streamlined workflow, which increases the laboratory’s efficiency. Being able to amplify directly from the Y-screen tray saves time and resources. 3 tables
Main Term(s): Forensic sciences
Index Term(s): Blood/body fluid analysis; DNA Typing; Efficiency; Investigative techniques; National Institute of Justice (NIJ); NIJ final report; Rape evidence kits; Rape investigations; Sexual assault
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