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NCJ Number: NCJ 240847     Find in a Library
Title: Development of a Real-Time Method to Detect DNA Degradation in Forensic Samples
  Document URL: HTML 
Author(s): Janice A. Nicklas, Ph.D. ; Trisha Noreault-Conti, Ph.D. ; Eric Buel, Ph.D.
  Journal: Journal of Forensic Sciences  Volume:57  Issue:2  Dated:March 2012  Pages:466 to 471
Date Published: 03/2012
Page Count: 6
  Annotation: This article describes the development of a Plexor® technology/real-time PCR DNA degradation detection assay
Abstract: Knowledge of the degradation state of evidentiary DNA samples would allow selection of the appropriate analysis method (standard short tandem repeats [STRs] vs. mini STRs vs. mtDNA). This article describes the development of a Plexor® technology/real-time PCR DNA degradation detection assay, which uses a common forward primer and two reverse primers (different fluorophores) to generate two Alu amplicons (63 and 246 bp). This very sensitive assay was optimized for reaction volume, cycle number, anneal/extend time, and temperature. Using DNA samples degraded with DNaseI, the ratio of the concentration of the short amplicon to the concentration of the long amplicon (degradation ratio) was increased versus time of degradation. Experiments were performed on a variety of environmentally degraded samples (age, sunlight, heat) and with seven commonly encountered forensic inhibitors. The degradation ratio was found to predict the observed loss of larger STR loci seen in the analysis of comprised samples. Abstract published by arrangement with John Wiley & Sons.
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Science and Technology ; Scientific techniques ; DNA fingerprinting ; NIJ grant-related documents
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2005-DA-BX-K003
Type: Report (Technical)
Country: United States of America
Language: English
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