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NCJ Number: NCJ 240849     Find in a Library
Title: Development of a Fast, Simple Profiling Method for Sample Screening Using High Resolution Melting (HRM) of STRs
Journal: Journal of Forensic Sciences  Volume:57  Issue:2  Dated:March 2012  Pages:478 to 488
Author(s): Janice A. Nicklas, Ph.D. ; Trisha Noreault-Conti, Ph.D. ; Eric Buel, Ph.D.
Date Published: 03/2012
Page Count: 11
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2005-DA-BX-K003
Document: HTML 
Type: Report (Technical)
Language: English
Country: United States of America
Annotation: This article examines a screening assay developed to provide preliminary individualization of crime scene samples thus eliminating expensive, time-consuming short tandem repeat (STR) profiling of nonprobative samples.
Abstract: A screening assay has been developed to provide preliminary individualization of crime scene samples thus eliminating expensive, time-consuming short tandem repeat (STR) profiling of nonprobative samples. High resolution melting performed in a real-time PCR instrument is used to detect the slight melting differences between the length and sequence variations of 22 forensic STRs. Three STRs (vWA, D18S51, THO1) were chosen to develop an assay which was optimized for Mg++ concentration, annealing/extension time/temperature, assay volume, and bovine serum albumin addition. The assay was tested for reproducibility, uniformity for genotype, melting profile consistency, effects of inhibitors, and mixture effects. The assay could be used to determine DNA concentration when a standard curve is run simultaneously. Calculations of costs show that the assay can save significant time and money for a crime with many samples or suspects. Abstract published by arrangement with John Wiley & Sons.
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Crime Laboratories (Crime Labs) ; Evidence identification and analysis ; DNA fingerprinting ; NIJ grant-related documents
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=262930

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