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NCJ Number: NCJ 241097     Find in a Library
Title: Developmental Validation of the AmpFlSTR® NGM SElect™ PCR Amplification Kit: A Next-Generation STR Multiplex with the SE33 Locus
Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:41 to 51
Author(s): Robert L. Green ; Robert E. Lagace ; Nicola J. Oldroyd ; Lori K. Hennessy ; Julio J. Mulero
Date Published: 01/2013
Page Count: 11
Publisher: http://www.elsevier.com 
Type: Report (Study/Research) ; Test/Measurement
Language: English
Country: United States of America
Annotation: The studies reported in this article demonstrated that the NGM SElect Kit provides equivalent overall performance to the NGM Kit, but with even greater discriminatory power due to the inclusion of the highly informative SE33 locus.
Abstract: The AmpFlSTR(®) NGM SElect™ PCR Amplification Kit is a new 17-plex STR genotyping kit designed for use primarily in forensic casework analysis. The kit was designed to be a counterpart to the AmpFlSTR(®) NGM™ Kit for laboratories wishing to add the SE33 locus to the new European Standard Set of STR loci. The NGM SElect Kit shares the same primer sets for 16 common loci with the NGM Kit (D10S1248, D3S1358, vWA, D16S539, D2S1338, amelogenin, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D22S1045, D2S441, D1S1656 and D12S391), with additional primers for the SE33 locus. Developmental validation studies followed the Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines for STR kit manufacturers and tested several critical areas of kit performance including a sensitivity series, DNA mixtures and inhibited samples. The studies demonstrated that the NGM SElect Kit provides equivalent overall performance to the NGM Kit, but with even greater discriminatory power due to the inclusion of the highly informative SE33 locus. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Suspect identification ; Investigative techniques ; Weapons identification ; DNA fingerprinting ; Instrument validation ; Parentage determination
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263185

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