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NCJRS Abstract

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  NCJ Number: NCJ 241097     Find in a Library
  Title: Developmental Validation of the AmpFlSTR® NGM SElect™ PCR Amplification Kit: A Next-Generation STR Multiplex with the SE33 Locus
  Author(s): Robert L. Green ; Robert E. Lagace ; Nicola J. Oldroyd ; Lori K. Hennessy ; Julio J. Mulero
  Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:41 to 51
  Date Published: 01/2013
  Page Count: 11
  Annotation: The studies reported in this article demonstrated that the NGM SElect Kit provides equivalent overall performance to the NGM Kit, but with even greater discriminatory power due to the inclusion of the highly informative SE33 locus.
  Abstract: The AmpFlSTR(®) NGM SElect™ PCR Amplification Kit is a new 17-plex STR genotyping kit designed for use primarily in forensic casework analysis. The kit was designed to be a counterpart to the AmpFlSTR(®) NGM™ Kit for laboratories wishing to add the SE33 locus to the new European Standard Set of STR loci. The NGM SElect Kit shares the same primer sets for 16 common loci with the NGM Kit (D10S1248, D3S1358, vWA, D16S539, D2S1338, amelogenin, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, D22S1045, D2S441, D1S1656 and D12S391), with additional primers for the SE33 locus. Developmental validation studies followed the Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines for STR kit manufacturers and tested several critical areas of kit performance including a sensitivity series, DNA mixtures and inhibited samples. The studies demonstrated that the NGM SElect Kit provides equivalent overall performance to the NGM Kit, but with even greater discriminatory power due to the inclusion of the highly informative SE33 locus. (Published Abstract)
  Main Term(s): Forensics/Forensic Sciences
  Index Term(s): Suspect identification ; Investigative techniques ; Weapons identification ; DNA fingerprinting ; Instrument validation ; Parentage determination
  Publisher URL: http://www.elsevier.com 
  Type: Report (Study/Research) ; Test/Measurement
  Country: United States of America
  Language: English
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263185

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