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NCJRS Abstract

The document referenced below is part of the NCJRS Library collection. To conduct further searches of the collection, visit the NCJRS Abstracts Database. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

 
  NCJ Number: NCJ 241102     Find in a Library
  Title: Developmental Validation of DogFiler, a Novel Multiplex for Canine DNA Profiling in Forensic Casework
  Document URL: HTML PDF 
  Author(s): Elizabeth Wictum ; Teri Kun ; Christina Lindquist ; Julia Malvick ; Dianne Vankan ; Benjamin Sacks
  Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:82 to 91
  Date Published: 01/2013
  Page Count: 10
  Annotation: This study examined DNA analysis on canine forensic samples.
  Abstract: While the analysis of human DNA has been the focus of large-scale collaborative endeavors, non-human forensic DNA analysis has not benefited from the same funding streams and coordination of effort. Consequently, the development of standard marker panels, allelic ladders and allele-specific sequence data comparable to those established for human forensic genetics has lagged. To meet that need for domestic dogs, the authors investigated sequence data provided by the published 7.6X dog genome for novel short tandem repeat markers that met the study's criteria for sensitivity, stability, robustness, polymorphic information content, and ease of scoring. Fifteen unlinked tetranucleotide repeat markers were selected from a pool of 3,113 candidate markers and assembled with a sex-linked marker into a multiplex capable of generating a full profile with as little as 60pg of nuclear DNA. An accompanying allelic ladder was assembled and sequenced to obtain detailed repeat motif data. Validation was carried out according to SWGDAM guidelines, and the DogFiler panel has been integrated into forensic casework and accepted in courts across the United States. Applying various formulae for calculating random match probabilities for inbred populations, estimates for this panel of markers have proven to be comparable to those obtained in human forensic genetics. The DogFiler panel and the associated allelic ladder represent the first published non-human profiling system to fully address all SWGDAM recommendations. (Published Abstract)
  Main Term(s): Forensics/Forensic Sciences
  Index Term(s): Suspect identification ; Investigative techniques ; DNA fingerprinting ; Animals (law enforcement) ; United States of America
  Sponsoring Agency: University of California, Davis Veterinary Genetics Laboratory
United States of America
  Publisher URL: http://www.elsevier.com 
  Type: Report (Study/Research) ; Test/Measurement
  Country: United States of America
  Language: English
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263190

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