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NCJ Number: NCJ 241103     Find in a Library
Title: High-Throughput Analysis Using AmpFlSTR Identifiler With the Applied Biosystems 3500xl Genetic Analyser
Author(s): Amanda Kirkham ; John Haley ; Yordina Haile ; Andrea Grout ; Colin Kimpton ; Ahmed Al-Marzouqi ; Peter Gill
  Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:92 to 97
Date Published: 01/2013
Page Count: 6
  Annotation: High throughput analysis of buccal scrape reference samples using the Identifiler system on the Applied Biosystems 3500xl Genetic Analyser is described.
Abstract: This platform is much more sensitive than previous platforms, e.g. 3130xl. The range of signal detection is also much greater and this means that the system is more tolerant to a wide range of input template concentrations. DNA quantity is not a limiting factor in the analysis of buccal scrapes, hence the entire analytical procedure (process) was designed around a target input of 1.5ng, to minimize low level DNA profiles and associated events of allele dropout. A universal stochastic threshold/limit of detection (LOD) of 300 relative fluorescent units (rfu) was applied across all loci. This level is twice that of comparable systems such as SGM Plus analyzed on 3130xl instruments. After analysis of dropout probabilities, heterozygous balance, drop-in and stutter characteristics, rule-sets were programmed into the FSS i-cubed software. The process has a success rate greater than 95 percent. No discordances or mis-designations occurred when applied to a validation set of more than 1,000 samples. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Victim identification ; Suspect identification ; Investigative techniques ; DNA fingerprinting ; Parentage determination
Grant Number: 285487
Publisher URL: 
Type: Report (Study/Research)
Country: United States of America
Language: English
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