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NCJ Number: NCJ 241106     Find in a Library
Title: Prevalence and Persistence of Male DNA Identified in Mixed Saliva Samples After Intense Kissing
  Document URL: HTML PDF 
Author(s): Natalia Kamodyova ; Jaroslava Durdiakova ; Peter Celec ; Tatiana Sedlackova ; Gabriela Repiska ; Barbara Sviezena ; Gabriel Minarik
  Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:124 to 128
Date Published: 01/2013
Page Count: 5
  Annotation: In this study, 12 voluntary pairs were tested at various intervals after intense kissing, and saliva samples were taken from the women to assess the presence of male DNA.
Abstract: Identification of foreign biological material by genetic profiling is widely used in forensic DNA testing in different cases of sexual violence, sexual abuse or sexual harassment. In all these kinds of sexual assaults, the perpetrator could constrain the victim to kissing. The value of the victim's saliva taken after such an assault has not been investigated in the past with currently widely used molecular methods of extremely high sensitivity (e.g. qPCR) and specificity (e.g. multiplex Y-STR PCR). In the current study, sensitivity-focused assays based on the SRY (single-copy gene) and DYS (multi-copy gene) sequence motifs confirmed the presence of male DNA in female saliva after 10 and even 60 min after kissing, respectively. For specificity, standard multiplex Y-STR PCR profiling was performed and male DNA was found in female saliva samples, as the entire Y-STR profile, even after 30 min in one sample. This study confirms that foreign DNA tends to persist for a restricted period of time in the victim's mouth, can be isolated from saliva after prompt collection and can be used as a valuable source of evidence. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Blood/body fluid analysis ; Sexual assault ; Investigative techniques ; DNA fingerprinting ; Foreign criminal justice research ; Czechoslovakia
Publisher URL: 
Type: Research (Applied/Empirical)
Country: United States of America
Language: English
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