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NCJ Number: NCJ 241107     Find in a Library
Title: Developmental Validation of the PowerPlex 18D System, a Rapid STR Multiplex for Analysis of Reference Samples
Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:129 to 135
Author(s): Kathryn Oostdik ; Julie French ; Donald Yet ; Briana Smalling ; Craig Nolde ; Peter M. Vallone ; Erica L.R. Butts ; Carolyn R. Hill ; Margaret C. Kline ; Theresa Rinta ; Stacey R. Allen ; Christopher K. Huber ; John Teske ; Benjamin Krenke ; Martin Ensenberger ; Patricia Fulmer ; Cynthia Sprecher ; Amy M. Gerow
Date Published: 01/2013
Page Count: 7
Publisher: http://www.elsevier.com 
Type: Report (Study/Research) ; Test/Measurement
Language: English
Country: United States of America
Annotation: In order to demonstrate suitability for human identification testing, the PowerPlex 18D System was tested for sensitivity, concordance, inhibitor tolerance, and performance with thermal cycling and reaction condition variation following SWWGDAM developmental validation guidelines.
Abstract: As short tandem repeat markers remain the foundation of human identification throughout the world, new STR multiplexes require rigorous testing to ensure the assays are sufficiently robust and reliable for genotyping purposes. The PowerPlex 18D System was created for the direct amplification of buccal and blood samples from FTA storage cards and reliably accommodates other sample materials. The PowerPlex 18D System allows simultaneous amplification of the 13 CODIS loci and amelogenin along with 4 additional loci: Penta E, Penta D, D2S1338, and D19S433. To demonstrate suitability for human identification testing, the PowerPlex 18D System was tested for sensitivity, concordance, inhibitor tolerance, and performance with thermal cycling and reaction condition variation following SWWGDAM developmental validation guidelines. Given these results, PowerPlex 18D System can confidently be used for forensic and human identification testing. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Victim identification ; Suspect identification ; Investigative techniques ; DNA fingerprinting ; Instrument validation ; Parentage determination
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263195

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