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NCJ Number: NCJ 241109     Find in a Library
Title: Genome-Wide mRNA Profiling and Multiplex Quantitative RT-PCR for Forensic Body Fluid Identification
Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:143 to 150
Author(s): Seong-Min Park ; Seong-Yeon Park ; Jeong-Hwan Kim ; Tae-Wook Kang ; Jong-Lyul Park ; Kwang-Man Woo ; Jong-Sik Kim ; Han-Chul Lee ; Seon-Young Kim ; Seung-Hwan Lee
Date Published: 01/2013
Page Count: 8
Publisher: http://www.elsevier.com 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: In forensic science, identifying a tissue where a forensic specimen was originated is one of the principal challenges.
Abstract: In forensic science, identifying a tissue where a forensic specimen was originated is one of the principal challenges. Messenger RNA (mRNA) profile clearly reveals tissue-specific gene expression patterns that many attempts have been made to use RNA for forensic tissue identification. To systematically investigate the body-fluid-specific expression of mRNAs and find novel mRNA markers for forensic body fluid identification, the authors performed DNA microarray experiment with 24 Korean body fluid samples. Shannon entropy and Q-values were calculated for each gene, and 137 body-fluid-specific candidate genes were selected. By applying more stringent criteria, the authors further selected 28 candidate genes and validated them by RT-PCR and qRT-PCR. As a result, the authors suggest a novel combination of four body-fluid-specific mRNA makers: PPBP for blood, FDCSP for saliva, MSMB for semen and MSLN for vaginal secretion. Multiplex qRT-PCR assay was designed using the four mRNA markers and DNA/RNA co-extraction method was tested for forensic use. This study will provide a thorough examination of body-fluid-specifically expressed mRNAs, which will enlarge the possibility of practical use of RNA for forensic purpose. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Blood/body fluid analysis ; Sexual assault ; Rape investigations ; DNA fingerprinting ; Foreign criminal justice research ; Korea (South)
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263197

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