skip navigation

CrimeSolutions.gov

Add your conference to our Justice Events calendar

PUBLICATIONS

NCJRS Abstract

The document referenced below is part of the NCJRS Library collection.
To conduct further searches of the collection, visit the NCJRS Abstracts Database.

How to Obtain Documents
 
NCJ Number: NCJ 241110     Find in a Library
Title: Developmental Validation of Mini-DogFiler for Degraded Canine DNA
Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:151 to 158
Author(s): Teri Kun ; Leslie A. Lyons ; Benjamin N. Sacks ; Ruth E. Ballard ; Christina Lindquist ; Elizabeth J. Wictum
Date Published: 01/2013
Page Count: 8
Publisher: http://www.elsevier.com 
Type: Report (Study/Research) ; Test/Measurement
Language: English
Country: United States of America
Annotation: Degraded human DNA analysis has benefited from the application of primer sets that amplify shorter nuclear sequences for core STR loci (miniSTRs), resulting in improved DNA profiles. This same approach was applied to this study's core canine STR loci.
Abstract: Dogs (Canis lupus familiaris) are kept as pets in 39 percent of American households and are, therefore, a significant source of potentially probative biological evidence. As with any biological evidence, degradation can occur as a consequence of environmental exposure causing fracturing of the DNA and a resulting loss of intact template. Degraded human DNA analysis has benefited from the application of primer sets that amplify shorter nuclear sequences for core STR loci (miniSTRs), resulting in improved DNA profiles. This same approach was applied to this study's core canine STR loci. The 16-locus "DogFiler" panel was redesigned into 3 panels of miniSTRs for analysis of degraded canine DNA, with all primer pairs producing amplicons below 205 base pairs in length. These new miniSTR marker panels - known as Mini-DogFiler - were validated according to SWGDAM guidelines, and concordance with the original 16-locus multiplex was demonstrated through genotyping 1,244 samples. The combination of these miniSTRs and a half-volume reaction increased the amplification success of degraded and low copy number canine biological samples resulting in a near three-fold increase in reportable alleles. This assemblage of miniSTRs along with the DogFiler panel and associated allelic ladder are the first non-human DNA profiling system to parallel the human forensic paradigm. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Evidence identification and analysis ; Investigative techniques ; DNA fingerprinting ; Animals (law enforcement) ; United States of America
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263198

* A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's web site is provided.