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NCJ Number: NCJ 241114     Find in a Library
Title: Quantitative Assessment of a Reliable Screening Technique for the STR Analysis of Telogen Hair Roots
Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:180 to 188
Author(s): Janette Edson ; Elizabeth M. Brooks ; Carolyn McLaren ; James Robertson ; Dennis McNevin ; Alan Cooper ; Jeremy J. Austin
Date Published: 01/2013
Page Count: 9
Sponsoring Agency: Australian Research Council
Australia
Grant Number: LP083333
Publisher: http://www.elsevier.com 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: Recent studies have revealed that telogen roots may be shed with adhering material that may contain cells, thus providing a potential source of nuclear DNA. A simple histological stain can be used to screen telogen roots for the presence of nuclei, thus increasing the chance of selecting roots that may yield nuclear DNA.
Abstract: Human telogen hairs are commonly recovered as trace evidence but currently have limited use for forensic DNA analysis. Recent studies have revealed that telogen roots may be shed with adhering material that may contain cells, thus providing a potential source of nuclear DNA. A simple histological stain can be used to screen telogen roots for the presence of nuclei, thus increasing the chance of selecting roots that may yield nuclear DNA. Using this method to visualise nuclei, the authors surveyed 998 hairs from 136 individuals, quantified the number of nuclei, extracted DNA and evaluated corresponding DNA yield and STR profiling success. Of the hairs screened, 35 percent of telogen roots contained nuclei and in total 6 percent of all roots screened had more than 100 nuclei. The number of nuclei associated with telogen roots was independent of the presence or absence of visibly adhering material, highlighting the importance of using histological staining rather than simple microscopic examination. DNA yield and STR profiling were significantly and positively correlated with nuclei number. The methods presented here can be incorporated into routine trace and DNA analysis providing an efficient and cost effective method to screen telogen hairs, and predict STR profiling success prior to destructive DNA analysis. The results of this study indicate telogen hairs may provide a reliable source of nuclear DNA for use in routine casework. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Victim identification ; Suspect identification ; Hair and fiber analysis ; Investigative techniques ; DNA fingerprinting ; Foreign criminal justice research ; Australia
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263202

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