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NCJ Number: NCJ 241116     Find in a Library
Title: Potential Use of DNA Barcoding for the Identification of Tobacco Seized From Waterpipes
Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:194 to 197
Author(s): Cindy Carrier ; Francois Cholette ; Camilo Quintero ; Chris Fulcher
Date Published: 01/2013
Page Count: 4
Publisher: http://www.elsevier.com 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: This research used DNA barcoding adopted in the authors'laboratory for the identification of tobacco (Nicotiana spp.) in moassel samples seized from "hookah bars."
Abstract: DNA barcoding was adopted in the authors'laboratory for the identification of tobacco (Nicotiana spp.) in moassel samples seized from "hookah bars." As recommended by the CBOL Plant Working Group, this study used a 2-locus combination of rbcL and matK as the plant barcode. As previously reported rbcL routinely produced high quality bi-directional reads but had a lower discriminating power than matK. It was much more difficult obtaining high quality bi-directional reads with matK possibly because of poor sample quality. DNA barcoding successfully identified tobacco in over 60 commercial tobacco moassel products. On the other hand, negative results (no amplification) or the identification of non-tobacco species were obtained from herbal moassel products. This study clearly demonstrates the practical utility of DNA barcoding beyond taxonomy. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Drug detection ; Plant analysis ; Investigative techniques ; DNA fingerprinting ; Tobacco use ; Foreign criminal justice research ; Canada
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263204

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