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NCJ Number: NCJ 241119     Find in a Library
Title: Prototype PowerPlex Y23 System: A Concordance Study
Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:204 to 208
Author(s): Carey Davis ; Jianye Ge ; Cynthia Sprecher ; Abirami Chidambaram ; Jonelle Thompson ; Margaret Ewing ; Patricia Fulmer ; Dawn Rabbach ; Douglas Storts ; Bruce Budowle
Date Published: 01/2013
Page Count: 5
Publisher: http://www.elsevier.com 
Type: Report (Study/Research) ; Test/Measurement
Language: English
Country: United States of America
Annotation: A total of 951 samples from 6 populations were typed to evaluate the Prototype PowerPlex Y23 kit and examine concordance for 17 of the loci that are in common with those that can be typed using the AmpFlSTR(®) Yfiler™ kit (Life Technologies, Carlsbad, CA).
Abstract: The Prototype PowerPlex(®) Y23 System (Promega Corporation, Madison, WI) is a polymerase chain reaction-based amplification kit that targets the 23 Y STR loci DYS19, DYS385a/b, DYS389I, DYS389II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438, DYS439, DYS448, DYS456, DYS458, DYS481, DYS533, DYS549, DYS570, DYS576, DYS635, DYS643, and Y-GATA-H4. A total of 951 samples from 6 populations were typed to evaluate the Prototype PowerPlex Y23 kit and examine concordance for 17 of the loci that are in common with those that can be typed using the AmpFlSTR(®) Yfiler™ kit (Life Technologies, Carlsbad, CA). A total of 16,167 loci were analyzed for each multiplex, and overall concordance was observed. Because of different kit designs, and although concordant for the genetic type, discordant calls can occur due to a deletion at the DYS448 locus. Users should take into consideration such nomenclature anomalies when comparing Y STR profiles. This new kit allows a large battery of Y STR loci to be analyzed using the same basic technologies already employed in forensic laboratories. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Victim identification ; Suspect identification ; Comparative analysis ; Investigative techniques ; DNA fingerprinting ; Instrument validation ; Parentage determination ; United States of America
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263207

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