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NCJ Number: NCJ 241120     Find in a Library
Title: PCR Inhibitor Removal Using the NucleoSpin® DNA Clean-Up XS Kit
Journal: Forensic Science International: Genetics  Volume:7  Issue:1  Dated:January 2013  Pages:209 to 213
Author(s): Korie L. Faber ; Eric C. Person ; William R. Hudlow
Date Published: 01/2013
Page Count: 5
Publisher: http://www.elsevier.com 
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: The NucleoSpin® DNA Clean-Up XS kit was optimized for the best possible DNA recovery and then evaluated for its ability to remove eight commonly encountered PCR inhibitors including: bile salt, collagen, hematin, humic acid, indigo, melanin, tannic acid and urea.
Abstract: Forensic evidence samples are collected from an unlimited variety of substrates, which may contain compounds known to inhibit the polymerase chain reaction (PCR). These PCR inhibitors are co-extracted with the DNA sample and can negatively affect the DNA typing results, which can range from partial to complete inhibition of the short tandem repeat (STR) PCR. One potential solution is to remove the PCR inhibitors from the extracts prior to the STR PCR with the NucleoSpin® DNA Clean-Up XS kit. The kit contains a NucleoSpin® XS silica column that has a special funnel design of thrust rings along with a very small silica membrane, which allows for sample elution in a small volume that is appropriate for use with current STR typing kits. The NucleoSpin® DNA Clean-Up XS kit was optimized for the best possible DNA recovery and then evaluated for its ability to remove eight commonly encountered PCR inhibitors including: bile salt, collagen, hematin, humic acid, indigo, melanin, tannic acid and urea. Each of these PCR inhibitors was effectively removed by the NucleoSpin® DNA Clean-Up XS kit as demonstrated by generating more complete STR profiles from the cleaned up inhibitor samples than from the raw inhibitor samples. (Published Abstract)
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Victim identification ; Suspect identification ; Investigative techniques ; DNA fingerprinting ; Instrument validation ; Parentage determination ; United States of America
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263208

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