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NCJ Number: NCJ 241144     Find in a Library
Title: Forensic Application of the Affymetrix Human Mitochondrial Resequencing Array
Journal: Forensic Science International: Genetics  Volume:1  Dated:2007  Pages:196 to 198
Author(s): P.M. Vallone ; J.P. Jakupciak ; M.D. Coble
Date Published: 2007
Page Count: 3
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2003-IJ-R-029
Type: Report (Study/Research)
Language: English
Country: United States of America
Annotation: This study assessed the potential of the Affumetrix GeneChip Mitochondrial Resequencing Array (version 2.0) for forensic applications.
Abstract: The increasing availability of commercial DNA array platforms is an attractive option for forensic analyses. One such platform is the Affymetrix GeneChip Mitochondrial Resequencing Array version 2.0. The GeneChip platform allows for sequencing of the entire mitochondrial genome by hybridization. The mitochondrial genome is amplified in three separate reactions, fragmented, and hybridized onto the array. Full genome sequencing results can typically be obtained in approximately 2 days. In the current assessment, a challenging sample was selected from the NIST U.S. population samples: African-American. This sample contained 63 differences from the rCRS as previously determined from dideoxy fluorescent sequencing experiments. The amount of DNA needed for the array is comparable to that required by fluorescent methods for sequencing the entire mitochondrial genome; however, the array does not provide full sequence coverage. Because of this relatively high sample requirement, the array may have restrictions for running a limited quantity of casework sample. Still, the platform should be useful in running family reference samples for the elucidation of SNPs in the coding region that will help resolve individuals. Comparisons between the GeneChip and traditional sequencing indicate the array platform had more difficulty calling instances of point heteroplasmy, insertions, deletions, poly C regions, and some closely spaced polymorphisms. Materials and methods for the GeneChip experiments are described in detail. 1 table and 6 references
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Victim identification ; Suspect identification ; Investigative techniques ; DNA fingerprinting ; Parentage determination ; NIJ grant-related documents
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263232

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