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NCJ Number: NCJ 241197     Find in a Library
Title: Study on the Effects of Degradation and Template Concentration on the Amplification Efficiency of the STR Miniplex Primer Sets
Author(s): Denise T. Chung B.S. ; Jiri Drabek Ph.D. ; Kerry L. Opel M.A. ; John M. Butler Ph.D. ; Bruce R. McCord Ph.D.
  Journal: Journal of Forensic Sciences  Volume:49  Issue:4  Dated:July 2004  Pages:733 to 740

Date Published: 07/2004
Page Count: 8
  Annotation: This study investigated the effects of degradation and template concentration on the amplification efficiency of the STR Miniplex primer sets.
Abstract: This study on the effects of degradation and template concentration on the amplification efficiency of the STR miniplex primer sets found that genotypes obtained from Big-Mini, Miniplex 2, and Miniplex 4 were that same with the genotypes obtained from the larger amplicons in the commercial kit. In addition, the study found that the Miniplex primer sets were able to produce complete profiles for all tested samples even for shorter template fragment lengths. The study also explored the sensitivity of 3 of the Miniplex primer sets with DNA concentrations ranging from 31 pg to 500 pg and found that two of the three primer sets were able to obtain correct genotypes at concentrations as low as 31 pg; this range of concentrations is below the range recommended for commercial sets. The primary objective of this study was to evaluate the effectiveness of a set of STR markers with redesigned primer sequences that produce smaller amplicons aimed at overcoming the problem of highly degraded forensic DNA samples obtained from crime scenes. Data for the study were obtained from analysis of enzymatically degraded DNA samples using commercially available kits and the newly developed STR Miniplex primer sets. The findings indicate that the Miniplex primer sets are capable of producing more complete DNA profiles when compared to larger amplicons obtained from the commercial kits, and that correct genotypes can be obtained with lower template concentrations than those recommended for commercial kits. These findings confirm the effectiveness of the redesigned primers at increasing the probability of obtaining a usable profile from degraded DNA samples compared to standard commercially available kits. Table, figures, and references
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Evidence identification and analysis ; Evidence ; DNA fingerprinting ; Forensic engineering ; NIJ grant-related documents
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2002-IJ-CX-K007
Type: Research (Applied/Empirical)
Country: United States of America
Language: English
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