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NCJ Number: NCJ 241198     Find in a Library
Title: Concordance Study Between Miniplex Assays and a Commercial STR Typing Kit
Journal: Journal of Forensic Sciences  Volume:49  Issue:4  Dated:July 2004  Pages:859 to 860

Author(s): Jiri Drabek, Ph.D. ; Denise T. Chung, B.S. ; John M. Butler, Ph.D. ; Bruce R. McCord, Ph.D.
Date Published: 07/2004
Page Count: 2
Sponsoring Agency: National Institute of Justice
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2002-IJ-CX-K007
Type: Research (Applied/Empirical)
Language: English
Country: United States of America
Annotation: This study investigated the concordance between Miniplex assays and a commercial STR typing kit.
Abstract: This study investigated the concordance between Miniplex assays and a commercial STR typing kit and found that of the 532 samples evaluated, full concordance was observed in 99.77 percent of STR allele calls compared (6,369 STR alleles out of a total of 6,384 STR alleles). For those alleles that were not in concordance, primer binding site mutations were identified and alterations were made to the Miniplex system to correct for discrepancies in future studies. Data for the study were obtained from analysis of 532 anonymous liquid blood samples. The samples were extracted, quantified, and typed using the Applied Biosystems AmpFLSTR Identifiler kit. In addition, Miniplex short tandem repeat (STR) assays were used and run on an ABI Prism 310 Genetic Analyzer. For each of the samples, a total of 12 STR loci were compared between the single amplification Identifiler it and the 3 separate Miniplex sets. The analysis found almost complete concordance between the two identification methods. Plans for future research are discussed. Table and references
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Evidence identification and analysis ; Forensic pathology ; DNA fingerprinting ; Forensic engineering ; NIJ grant-related documents
   
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https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263288

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