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NCJ Number: NCJ 241213     Find in a Library
Title: STR Melting Curve Analysis as a Genetic Screening Tool for Crime Scene Samples
  Document URL: HTML 
Author(s): Quang Nguyen, B.S. ; Jason McKinney, Ph.D. ; Donald J. Johnson, M.S. ; Katherine A. Roberts, Ph.D. ; Winters R. Hardy, Ph.D.
  Journal: Journal of Forensic Sciences  Volume:57  Issue:4  Dated:July 2012  Pages:887 to 899
Date Published: 07/2012
Page Count: 13
  Annotation: In this proof-of-concept study, high-resolution melt curve (HRMC) analysis was investigated as a postquantification screening tool to discriminate human CSF1PO and THO1 genotypes amplified with mini-STR primers in the presence of SYBR Green or LCGreen Plus dyes.
Abstract: In this proof-of-concept study, high-resolution melt curve (HRMC) analysis was investigated as a postquantification screening tool to discriminate human CSF1PO and THO1 genotypes amplified with mini-STR primers in the presence of SYBR Green or LCGreen Plus dyes. A total of 12 CSF1PO and 11 HUMTHO1 genotypes were analyzed on the LightScanner HR96 and LS-32 systems and were correctly differentiated based upon their respective melt profiles. Short STR amplicon melt curves were affected by repeat number, and single-source and mixed DNA samples were additionally differentiated by the formation of heteroduplexes. Melting curves were shown to be unique and reproducible from DNA quantities ranging from 20 to 0.4 ng and distinguished identical from nonidentical genotypes from DNA derived from different biological fluids and compromised samples. Thus, a method is described which can assess both the quantity and the possible probative value of samples without full genotyping. Abstract published by arrangement with John Wiley & Sons.
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Crime Laboratories (Crime Labs) ; Crime scene ; Criminalistics ; Scientific techniques ; DNA fingerprinting
Type: Research (Applied/Empirical)
Country: United States of America
Language: English
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263303

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