skip navigation

CrimeSolutions.gov

Add your conference to our Justice Events calendar

PUBLICATIONS

NCJRS Abstract

The document referenced below is part of the NCJRS Library collection.
To conduct further searches of the collection, visit the NCJRS Abstracts Database.

How to Obtain Documents
 
NCJ Number: NCJ 241217     Find in a Library
Title: Trace Detection of Meglumine and Diatrizoate from Bacillus Spore Samples Using Liquid Chromatography/Mass Spectrometry
Journal: Journal of Forensic Sciences  Volume:57  Issue:4  Dated:July 2012  Pages:923 to 931
Author(s): Catherine Swider, B.S. ; Kelly Maguire, M.S. ; Michael Rickenbach, Ph.D. ; Madeline Montgomery, B.S. ; Matthew J. Ducote, Ph.D. ; Craig A. Marhefka, Ph.D.
Date Published: 07/2012
Page Count: 9
Document: HTML 
Type: Report (Technical)
Language: English
Country: United States of America
Annotation: This article discusses the purification of B. anthracis spores.
Abstract: Following the September 11, 2001 terrorist attacks, letters containing Bacillus anthracis were distributed through the United States postal system killing five people. A complex forensic investigation commenced to identify the perpetrator of these mailings. A novel liquid chromatography/mass spectrometry protocol for the qualitative detection of trace levels of meglumine and diatrizoate in dried spore preparations of B. anthracis was developed. Meglumine and diatrizoate are components of radiographic imaging products that have been used to purify bacterial spores. Two separate chromatographic assays using multiple mass spectrometric analyses were developed for the detection of meglumine and diatrizoate. The assays achieved limits of detection for meglumine and diatrizoate of 1.00 and 10.0 ng/mL, respectively. Bacillus cereus T strain spores were effectively used as a surrogate for B. anthracis spores during method development and validation. This protocol was successfully applied to limited evidentiary B. anthracis spore material, providing probative information to the investigators. Abstract published by arrangement with John Wiley & Sons.
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Chromatography ; Trace evidence ; Evidence identification and analysis ; Mass spectroscopy ; Chemical irritants ; Domestic terrorism ; Chemical Weapons
   
  To cite this abstract, use the following link:
https://www.ncjrs.gov/App/Publications/abstract.aspx?ID=263307

* A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's web site is provided.