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NCJ Number: NCJ 241234     Find in a Library
Title: AutoMate Express™ Forensic DNA Extraction System for the Extraction of Genomic DNA from Biological Samples
  Document URL: HTML 
Author(s): Jason Y. Liu, Ph.D. ; Chang Zhong, Ph.D. ; Allison Holt, Ph.D. ; Robert Lagace, B.S. ; Michael Harrold, M.S. ; Alan B. Dixon, M.S.F.S. ; Maxim G. Brevnov, Ph.D. ; Jaiprakash G. Shewale, Ph.D. ; Lori K. Hennessy, Ph.D.
  Journal: Journal of Forensic Sciences  Volume:57  Issue:4  Dated:July 2012  Pages:1022 to 1030
Date Published: 07/2012
Page Count: 9
  Annotation: This study examined the AutoMate Express™ Forensic DNA Extraction System that was developed for automatic isolation of DNA from a variety of forensic biological samples.
Abstract: The AutoMate Express™ Forensic DNA Extraction System was developed for automatic isolation of DNA from a variety of forensic biological samples. The performance of the system was investigated using a wide range of biological samples. Depending on the sample type, either PrepFiler™ lysis buffer or PrepFiler BTA™ lysis buffer was used to lyse the samples. After lysis and removal of the substrate using LySep™ column, the lysate in the sample tubes were loaded onto AutoMate Express™ instrument and DNA was extracted using one of the two instrument extraction protocols. This study showed that DNA was recovered from as little as 0.025 uL of blood. DNA extracted from casework-type samples was free of detectable PCR inhibitors and the short tandem repeat profiles were complete, conclusive, and devoid of any PCR artifacts. The system also showed consistent performance from day-to-day operation. Abstract published by arrangement with John Wiley & Sons.
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Biological influences ; Blood/body fluid analysis ; Scientific techniques ; DNA fingerprinting ; Instrument validation
Type: Research (Applied/Empirical)
Country: United States of America
Language: English
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