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NCJ Number: NCJ 241735     Find in a Library
Title: Bone DNA Extraction and Purification Using Silica-Coated Paramagnetic Beads
  Document URL: HTML 
Author(s): M. J. Dukes ; A. L. Williams ; C. M. Massey ; P. W. Wojtkiewicz
  Journal: American Journal of Physical Anthropology  Volume:148  Issue:3  Dated:July 2012  Pages:473 to 482
Date Published: 07/2012
Page Count: 10
  Annotation: The goal of this study was to develop a simple method to improve DNA recovery from challenging bone samples.
Abstract: The goal of this study was to develop a simple method to improve DNA recovery from challenging bone samples. To this end, an optimized procedure was developed that combined the demineralization and DNA extraction into a single step, followed by DNA purification using an automated silica-coated paramagnetic bead procedure. This method replaced a previous silica-membrane-based procedure, which was able to recover sufficient DNA to obtain full autosomal and Y chromosome STR profiles from greater than 90 percent of the samples, including samples greater than 20 years old. The development process began with the evaluation of buffer and demineralization systems to determine the best reagent combination. During the developmental process, we observed that the addition of EDTA and DTT affected silica-based DNA purification methods by raising the pH of the digest buffer. The protocols with buffer ATL, PK, EDTA, and DTT followed by lowering the pH with sodium acetate just before purification resulted in the best yields. The method reduced the extraction volume from 10 to 1.5 ml and used commercially available reagents already being utilized in forensic DNA casework. Because of the simplicity and small volume needed for the procedure, many steps where contamination could be introduced have been eliminated or minimized. This study demonstrated a new method of recovering DNA from bone samples capable of extracting trace quantities of DNA, removing potential inhibitors, and minimizing the potential for exogenous DNA contamination. Abstract published by arrangement with John Wiley & Sons.
Main Term(s): Forensics/Forensic Sciences
Index Term(s): Research methods ; Bone analysis ; Scientific techniques ; DNA fingerprinting ; NIJ grant-related documents
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2006-DN-BX-K103
Type: Report (Technical)
Country: United States of America
Language: English
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