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NCJRS Abstract

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  NCJ Number: NCJ 241736     Find in a Library
  Title: Determination of Tissue-Specific DNA Methylation Patterns in Forensic Biofluids Using Bisulfite Modification and Pyrosequencing
  Document URL: HTML 
  Author(s): T. Madi ; K. Balamurugan ; R. Bombardi ; G. Duncan ; B. McCord
  Journal: Electrophoresis  Volume:33  Issue:12  Dated:July 2012  Pages:1736 to 1745
  Date Published: 07/2012
  Page Count: 10
  Annotation: The goal of this study is to explore the application of epigenetic markers in the identification of biofluids that are commonly found at the crime scene.
  Abstract: The goal of this study is to explore the application of epigenetic markers in the identification of biofluids that are commonly found at the crime scene. A series of genetic loci were examined in order to define epigenetic markers that display differential methylation patterns between blood, saliva, semen, and epithelial tissue. Among the different loci tested, we have identified a panel of markers, C20orf117, ZC3H12D, BCAS4, and FGF7, that can be used in the determination of these four tissue types. Since methylation modifications occur at cytosine bases that are immediately followed by guanine bases (CpG sites), methylation levels were measured at CpG sites spanning each marker. Up to 11 samples of each tissue type were collected and subjected to bisulfite modification to convert unmethylated CpG-associated cytosine bases to thymine bases. The bisulfite modified DNA was then amplified via nested PCR using a primer set of which one primer was biotin labeled. Biotinylated PCR products were in turn analyzed and the methylation level at each CpG site was quantitated by pyrosequencing. The percent methylation values at each CpG site were determined and averaged for each tissue type. The results indicated significant methylation differences between the tissue types. The methylation patterns at the ZC3H12D and FGF7 loci differentiated sperm from blood, saliva, and epithelial cells. The C20orf117 locus differentiated blood from sperm, saliva, and epithelial cells and saliva was differentiated from blood, sperm, and epithelial cells at a fourth locus, BCAS4. The results of this study demonstrate the applicability of epigenetic markers as a novel tool for the determination of biofluids using bisulfite modification and pyrosequencing. Abstract published by arrangement with John Wiley & Sons.
  Main Term(s): Forensics/Forensic Sciences
  Index Term(s): Blood/body fluid analysis ; Crime scene ; Tissue analysis ; DNA fingerprinting ; NIJ grant-related documents
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2009-D1-BX-0293
  Type: Report (Technical)
  Country: United States of America
  Language: English
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