skip navigation

Justinfo Subscribe to Stay Informed

Add your conference to our Justice Events calendar


NCJRS Abstract


Subscribe to Stay Informed
Want to be in the know? JUSTINFO is a biweekly e-newsletter containing information about new publications, events, training, funding opportunities, and Web-based resources available from the NCJRS Federal sponsors. Sign up to get JUSTINFO in your inbox.

The document referenced below is part of the NCJRS Library collection.
To conduct further searches of the collection, visit the NCJRS Abstracts Database.

How to Obtain Documents
NCJ Number: NCJ 242695     Find in a Library
Title: Deep-Sequencing Technologies and Potential Applications in Forensic DNA Testing
Author(s): R. R. Zascavage ; S. J. Shewale ; J.V. Planz
  Journal: Forensic Science Review  Volume:25  Issue:1 & 2  Dated:March 2013  Pages:79 to 105
Date Published: 03/2013
Page Count: 27
  Annotation: This article discusses development of second- and third-generation DNA sequencing technologies.
Abstract: Development of second- and third-generation DNA sequencing technologies have enabled an increasing number of applications in different areas, such as molecular diagnostics, gene therapy, monitoring food and pharmaceutical products, biosecurity, and forensics. These technologies are based on different biochemical principles such as monitoring released pyrophosphate upon incorporation of a base (pyrosequencing), fluorescence detection subsequent to reversible incorporation of a fluorescently labeled terminator base, ligation-based approach wherein fluorescence of cleaved nucleotide after ligation is measured, measuring the proton released after incorporation of a base (semiconductor-based sequencing), monitoring incorporation of a nucleotide by measuring the fluorescence of the fluorophore attached to the phosphate chain of the nucleotide, and by detecting the altered charge in a protein nanopore due to released nucleotide by exonuclease cleavage of a DNA strand. Analysis of multiple DNA fragments in parallel increases the depth of coverage while decreasing labor, cost, and time, highlighting some major advantages of deep-sequencing technologies. DNA sequencing has been routinely used in the forensic laboratories for mitochondrial DNA analysis. Fragment analysis, however, is the preferred method for Short Tandem Repeat genotyping due to the cumbersome and costly nature of first-generation DNA analysis. Studies include STR analysis to reveal hidden variation in the repeat regions, mtDNA sequencing, Single Nucleotide Polymorphism analysis, mixture resolution, and body fluid identification. Recent publications reveal that attempts are being made to expand the capability. (Published Abstract)
Main Term(s): Criminology
Index Term(s): Victim identification ; Suspect identification ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; Parentage determination
Type: Issue Overview
Country: Taiwan
Language: English
  To cite this abstract, use the following link:

* A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's web site is provided.