skip navigation

Add your conference to our Justice Events calendar


Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Library collection. To conduct further searches of the collection, visit the NCJRS Abstracts Database. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

  NCJ Number: NCJ 242695     Find in a Library
  Title: Deep-Sequencing Technologies and Potential Applications in Forensic DNA Testing
  Author(s): R. R. Zascavage ; S. J. Shewale ; J.V. Planz
  Journal: Forensic Science Review  Volume:25  Issue:1 & 2  Dated:March 2013  Pages:79 to 105
  Date Published: 03/2013
  Page Count: 27
  Annotation: This article discusses development of second- and third-generation DNA sequencing technologies.
  Abstract: Development of second- and third-generation DNA sequencing technologies have enabled an increasing number of applications in different areas, such as molecular diagnostics, gene therapy, monitoring food and pharmaceutical products, biosecurity, and forensics. These technologies are based on different biochemical principles such as monitoring released pyrophosphate upon incorporation of a base (pyrosequencing), fluorescence detection subsequent to reversible incorporation of a fluorescently labeled terminator base, ligation-based approach wherein fluorescence of cleaved nucleotide after ligation is measured, measuring the proton released after incorporation of a base (semiconductor-based sequencing), monitoring incorporation of a nucleotide by measuring the fluorescence of the fluorophore attached to the phosphate chain of the nucleotide, and by detecting the altered charge in a protein nanopore due to released nucleotide by exonuclease cleavage of a DNA strand. Analysis of multiple DNA fragments in parallel increases the depth of coverage while decreasing labor, cost, and time, highlighting some major advantages of deep-sequencing technologies. DNA sequencing has been routinely used in the forensic laboratories for mitochondrial DNA analysis. Fragment analysis, however, is the preferred method for Short Tandem Repeat genotyping due to the cumbersome and costly nature of first-generation DNA analysis. Studies include STR analysis to reveal hidden variation in the repeat regions, mtDNA sequencing, Single Nucleotide Polymorphism analysis, mixture resolution, and body fluid identification. Recent publications reveal that attempts are being made to expand the capability. (Published Abstract)
  Main Term(s): Criminology
  Index Term(s): Victim identification ; Suspect identification ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting ; Parentage determination
  Type: Issue Overview
  Country: Taiwan
  Language: English
  To cite this abstract, use the following link:

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.