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Estimation of Postmortem Interval Using Kinetic Analysis of the Third Component of Complement (C3) Cleavage

NCJ Number
115496
Journal
Journal of Forensic Sciences Volume: 34 Issue: 1 Dated: (January 1989) Pages: 207-217
Author(s)
Y Kominato; K Kumada; K Yamazaki; S Misawa
Date Published
1989
Length
11 pages
Annotation
To estimate postmortem interval (PMI), spontaneous cleavage of the third component of complement (C3) was studied in aged blood and cadaveric blood by crossed immunoelectrophoresis.
Abstract
Using the kinetics of C3 cleavage in vitro described as 'dC/dt = -kC', where C is the concentration of native C3 at time t and k is a first-order rate constant, Arrhenius' equation, and another equation which assumes a linear drop of body temperature after death, the percentages of C3 cleavage were calculated. There was a significant positive correlation between the calculated percentages and the measured percentages of up to 10 percent in cadaveric blood. We found that the comparison between the calculated percentage of C3 cleavage for each optional postmortem interval and the measured percentage of up to ten percent in cadaveric blood leads to the estimation of PMI. This approach is one step towards the development of an accurate method for determining PMI based on C3 cleavage, that is, on a first-order reaction. (Author abstract)