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Comparison of Abbott Fluorescence Polarization Immunoassay (FPIA) and Roche Radioimmunoassay for the Analyses of Cannabinoids in Urine Specimens

NCJ Number
136033
Journal
Journal of Forensic Sciences Volume: 37 Issue: 2 Dated: (March 1992) Pages: 632-635
Author(s)
W T Budgett; B Levine; A Xu; M L Smith
Date Published
1992
Length
4 pages
Annotation
Abbott fluorescence polarization immunoassay (FPIA) and Roche Abuscreen radioimmunoassay (RIA) were compared qualitatively with 142 urine specimens that contained 11-nor-delta-9-tetrahydrocannabinol-9-carboxylic acid, the major urinary metabolite of marijuana.
Abstract
A total of 142 specimens were analyzed by FPIA and RIA using the 100-ng/ml cut-off. The samples represented a range of concentrations above and below this administrative screening cut-off. Of the 142 specimens tested, 132 generated the same qualitative results. All specimens negative by FPIA and positive by RIA were within 20 percent of the cut-off, and seven of the nine specimens were within 10 percent of the cut-off. Similarly, the RIA counts per minute of the specimens negative by RIA and positive by FPIA were within 10 percent of the counts per minute produced by the cut-off calibrator. These data suggest that the RIA antibody might have slightly broader specificity in screening for cannabinoids; however, this slight difference is not significant. The study concludes that either of these immunoassays could be used singularly in a mass drug-screening laboratory and generate comparable results. 1 table, 1 figure, and 3 references

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