The current study explored the nondestructive nature of passive headspace separation using the charcoal strip and evaluated the significance of headspace concentration in fire debris analysis. The adsorption material used in the separation was a charcoal-impregnated binder. Gas chromatographic analysis was performed using a gas chromatograph fitted with a flame ionization detector. Twelve samples selected for study came from an actual case previously separated by passive headspace concentration and determined to be positive for the presence of a flammable or combustible liquid. Samples in the first group were each separated four times, while samples in the second group were separated at time intervals of 90 and 180 days from the original separation date. Samples in the third group were separated 180 and 365 days after the initial separation date, and samples in the fourth group were separated after 365. Results demonstrated that multiple seaprations by passive headspace could be performed without jeopardizing the recovery and identification of volatile residues. The only significant changes induced by the separation process seemed to occur in light residues, such as slightly weathered gasoline. 11 references and 15 figures