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Rapid Dot-Blot Method for Species Identification of Bloodstains

NCJ Number
141750
Journal
Journal of Forensic Sciences Volume: 38 Issue: 2 Dated: (March 1993) Pages: 448-454
Author(s)
S Matsuzawa; H Kimura; Y Itoh; H Wang; T Nakagawa
Date Published
1993
Length
7 pages
Annotation
A simple and rapid test method for the species identification of bloodstains is described; the test has many useful applications for forensic scientists and clinical laboratories.
Abstract
In this test, extracts of bloodstains were applied to a synthetic porous membrane and dried. The membrane was then quenched with glycine buffered saline containing BSA and Tween 20. A suspension mixture of colloidal gold particles (GP) coated with rabbit antiserum to human IgG was poured ont, gently whirled, and aspirated through the membrane. While spots from the human and monkey bloodstains became red, those from other species remained unstained. The antibody-coated GP reagent was prepared within 20 minutes using a small sample of antiserum and the actual test took between 3 and 4 minutes. Cellulose acetate membranes of 0.45 um or more in pore size were appropriate to the test. While the specificity of this test is inferior to that of the agglutination-inhibition test using antigen-coated latex particles, it is comparable to the methods using antibody-coated latex particles, ELISA, and the ring interfacial test. 3 tables, 2 figures, and 13 references

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