Randomly collected blood samples from 100 Arabs and 100 Pakistanis residing in Abu Dhabi were analyzed using the HLA-DQ and polymarker (LDLR, GC, GYPA, HBGG, D7S8) PCR based reverse dot blot systems. Allelic frequencies for each allele and observed heterozygosity for each locus were calculated. Departures from Hardy-Weinberg expectations (HWE) were determined using the unbiased estimate of the expected homozygote/heterozygote frequencies, the likelihood ratio test and the exact test. No significant departures from HWE expectations were detected. These homogeneity analyses are an example of how statistics can detect significant differences between populations and yet, if either population was used as a reference database, the resulting DNA profile frequency would not be substantially different for most practical forensic applications. The data support that these databases can be used for estimating the rarity of DNA profile frequencies. Moreover, the data may be useful for population genetics studies. Tables, figures, references