A total of 100 unrelated individuals from each group were genotyped for four independently assorting loci: HLA DQA1, CSF1PO, TPOX, and TH01. Allele frequencies were calculated; one-locus and two-locus tests for association were conducted; and the samples were compared by contingency table tests and F-statistic estimation. Although there is expected to be some genetic divergence between the two groups, forensic needs may be satisfied with a single Pakistani database of DNA profiles. The current data suggest that nine independently assorting loci will be sufficient to provide estimated profile probabilities of the order of 10 with an exponent of -9, but a set of 13 loci, as used in the United States, would better compensate for the dependencies introduced by family membership and evolutionary history. Thus, the study suggests that Araeen and Raajpoot data from Lahore City can be used to estimate probabilities for either group and reinforces the belief that any published frequencies for human identification loci can be used for forensic purposes in Pakistan populations, inside or outside that country, provided a sufficiently high value of the population structure parameter 0 is used. 4 tables and 12 references