This Type II restriction enzyme is extracted from the bacterium Haemophiliys aegyptius and recognizes the cleavage sequence GG^CC in single-stranded DNA. At 10 of the 13 AmpFlSTR loci, the authors demonstrated amplification of known and questioned DNA samples that were digested by the HaeIII restriction enzyme and stored at minus 70 degrees C for up to 8 years. The aim was successful STR amplification of digested DNA from known, high quality bloodstains and successful STR amplification of forensic samples historically processed for RFLP analysis. Full profiles were obtained using the 9-locus Profiler Plus multiplex system. In both the 9-locus Profiler and the 6-locus COfiler systems, only TH01, TPOX, and CSF1PO failed to amplify. Amelogenin successfully amplified in all three systems. The authors conclude it is possible, with minimal additional sample manipulation, to use archived, digested DNA in order to expand the locus information of unsolved RFLP cases to include STR systems currently in use in Canada and the United States. 12 references and 4 figures