The identification of human blood is considered very important in the practice of criminal investigation. Methods that are species-specific and highly sensitive usually require special laboratory equipment. In order to develop a method that is specific, sensitive, and convenient for use at the crime scene, a sandwich-hybridization method was applied for human blood identification. The test kit uses anti-human hemoglobin (Hb) monoclonal antibody. This antibody showed high species specificity and could detect as little as 20 ng human Hb. Cross activity was observed only by the baboon. It was able to detect dilutions up to 5,000,000 times and identify a 15.5 year-old human bloodstain. Because the sandwich-hybridization method is rapid (2 minutes) and does not require special equipment, it is considered useful for crime scene investigation. Tables