In order to increase the discriminating power of DNA analysis in forensic science, the study designed a new triplex short tandem repeat (STR) using three novel STR loci previously reported: D14S299 (wglc5), D15S233 (wgld1), and 9q2h2. The new system, designated a CDH triplex system, showed a high discriminating power, especially in Caucasians. The system is composed of three STR loci showing only regular tetranucleotide repeat alleles. The study enlarged the databases mainly of Japanese using this system, and compared them with databases of Caucasian and Chinese. Since all three loci have only regular tetranucleotide repeat alleles, each with high discriminating power, and have non-overlapping allele size ranges, the system can accurately type their alleles by electrophoresis on a denaturing polyacrylamide gel followed by silver staining in which the initial cost performance is lower than the fluorescent format. The study concludes that the CDH triplex system appears to be useful as a supplementary tool in forensic practice. Figure, tables, references