The Law Enforcement and Corrections Standards and Testing Program of the National Institute of Justice responds to the mandate of the Justice System Improvement Act of 1979 which directs the National Institute of Justice (NIJ) to encourage research and development to improve the criminal justice system and disseminate the results to Federal, State, and local agencies. The testing program determines the technological needs of justice system agencies, sets minimum performance standards for specific devices, tests commercially available equipment against those standards, and disseminates the standards the test results, both nationally and internationally. This report is the NIJ test protocol for comparative evaluation of protective gloves for use by law enforcement, corrections, and military personnel. The scope of the test protocol was limited to the types of gloves that met the rating levels described (biological hazards, cut resistant, and puncture resistant). The performance criteria that the protective gloves were evaluated against include: pathogenic resistance, dexterity, cut resistance, tear resistance, and puncture resistance. The quality assurance provisions included: responsibility for inspection; manufacturer quality assurance plan; examination procedure; test procedures; and test results reporting. Manufacturers are prohibited from placing any statement on the model of protective glove stating or suggesting that the model has been tested in accordance with the NIJ Test Protocol for Protective Gloves until the model has successfully completed testing at a NIJ-approved testing facility for the purposes of this test protocol.