Three adult males and three adult females defecated, without also urinating, into a clean bedpan. A questionnaire completed by each subject obtained information on diet, bowel habits, and general health. Each subject also provided a buccal swabbing as a DNA reference sample. The stool specimens and buccal swabs were submitted to the laboratory, without prior refrigeration or freezing, between 2 and 12 hours after collection. This report describes the laboratory sample preparation, blood and microscopic examination, organic extraction, the QIAamp DNA Stool Mini Kit extractions and modifications, DNA quantitation, STR amplification, and STR-typing. Modifications to the QIAGEN procedures resulted in the recovery of DNA from all samples for five of the six subjects under the five environmental conditions applied to the samples. DNA quantitation was obtained for the five subjects. The modifications of the QIAGEN needed for recovery of the DNA are described in this report. The authors conclude that although the kit may require modification to interface with specific applications, its cost, ease-of-use, and final product make it an efficient and effective forensic tool. 4 tables and 19 references