The tribes were Chenchu (n=100), Lambadi (n=107), Naikpod Gond (n=104), and Yerukula (n=101). Peripheral blood samples were obtained from consenting healthy, unrelated individuals of the four tribes. Genomic DNA was extracted from blood using the standard phenol-chloroform extraction method, followed by ethanol precipitation. Extracted DNA was quantitated with the Quantiblot Human DNA Quantitation Kit. The 15 tetranucleotides were D5S818, FGA, TPOX, CSF1PO, D3S1358, THO1, D13S317, D16S539, vWA, D8S1179, D21S11, D7S820, D2S1338, D19S433, and D18S51. The two pentanucleotides loci were Penta D and Penta E. Among the four tribal populations, these genetic markers are highly variable and hence ideal for human identification and analysis of genetic diversity in populations. The current analysis is consistent with the database of allele frequencies of polymorphic loci of other studied Indian populations. 4 tables and 15 references