DNA-based techniques for the identification of individuals are becoming increasingly relied upon in forensic science. The DNA fingerprinting method that the FBI and the British courts have accepted for use in identification of an individual is based on tandem repeats present in the human genome. The loci frequently used in forensic science consist of STRs (short tandem repeats) since they are readily amenable to PCR amplification. In addition to STR analysis, DNA profiling methods using microarrays are in their infancy and are desirable since they are compatible with miniaturized devices that could provide a high degree of speed, sensitivity, and portability, important features in forensic analysis. This study describes the technique, variable-length probe array (VLPA) in identifying and distinguishing between different DNA sequences using STR analysis and DNA microarrays. VLPA determines the length of an unknown STR using two novel innovations, a clamp sequence to ensure proper hybridization of the repeat sequences and a nuclease treatment to remove single-stranded DNA from the array. This method utilizes current widely available microarray technology and should allow for rapid determination of individual identity. This study demonstrates the feasibility of this new technique in a wide variety of applications that use STR analysis. Tables, figures, references