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NCJ Number: NCJ 212869   Add to Shopping cart   Find in a Library
Title: Increasing the Predictability and Success Rate of Skeletal Evidence Typing: Using Physical Characteristics of Bone as a Metric for DNA Quality and Quantity
  Document URL: PDF 
Author(s): Dr. David Foran PI
Corporate Author: Michigan State University
United States of America
Date Published: 02/2006
Page Count: 104
  Annotation: Donated skeletal specimens of some 20 individuals who had been buried in a small church cemetery between 1833 and 1861 were examined to determine the relationship between bone weathering and bone DNA degradation.
Abstract: The study found little relationship between the outward appearance of a bone and its DNA quantity or quality. The bone type, however, was a significant factor in the quality of the findings from DNA analysis. Long bones contained more DNA for analysis than other bones, and they were more likely to produce usable DNA results than ribs. Ribs, in turn, were more likely to produce usable DNA results than flat bones. The amount of DNA recovered was not significant in DNA amplification. Neither sex nor age were related to the success of DNA typing. An associated research project found that soil pH influenced bone preservation. Findings related to the DNA analysis included the determination that PCR inhibition significantly influenced quantitative PCR results; this was somewhat remedied by the addition of BSA. Amplification that used primers moved in toward the target sequence (Mini-STR's) made little difference in the ability to amplify nuclear DNA, but the use of nested PCR did weaken amplification. Whole genome amplification was of little use with the aged skeletal material. Quantitative PCR was used to measure the amount of mitochondrial DNA in each bone. 30 figures, 8 tables, and a 114-item bibliography
Main Term(s): Victim identification
Index Term(s): Bone analysis ; Forensics/Forensic Sciences ; Investigative techniques ; Death investigations ; DNA fingerprinting ; NIJ grant-related documents
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2002-IJ-CX-K016
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America
Type: Report (Study/Research)
Country: United States of America
Language: English
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