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NCJRS Abstract

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  NCJ Number: NCJ 214166   Add to Shopping cart   Find in a Library
  Title: Assessment and "In Vitro" Repair of Damaged DNA Templates
  Document URL: PDF 
  Author(s): Jack Ballantyne
  Corporate Author: National Ctr for Forensic Science
United States of America
  Date Published: 2006
  Page Count: 93
  Annotation: This study first examined the types of DNA damage encountered in forensically relevant strains of DNA and then attempted to repair the damage using in vitro repair systems.
  Abstract: Findings indicated that the main causes of the damage resulting in DNA profile loss were single and double strand breaks and modifications to the DNA structure. The in vitro DNA repair attempts, which focused on single strand break/gap repair and translesion synthesis assays, resulted in genetic signatures being recovered from previously intractable samples. Research methods used to examine types of DNA damage focused on the detection of damage caused by exogenous, environmental sources, UV irradiation, heat, humidity, and microorganism growth. Physiological DNA samples, both hydrated and dehydrated samples were subjected to varying doses of UVC and the extent of damage was assessed by gel electrophoresis and then by amplifying the sample using a standard autosomal STR (short tandem repeat) multiplex. The author also examined the effects of various laboratory storage conditions on the DNA in bloodstains. Bloodstains were either allowed to dry overnight or were placed in storage while still wet. Both sets of bloodstains were stored at room temperature for up to 4 years. Results indicated that bloodstains that were allowed to dry prior to storage were stable and showed no signs of degradation or loss of genetic profile. Tables, references, appendixes
  Main Term(s): Forensics/Forensic Sciences ; DNA fingerprinting
  Index Term(s): Blood stains ; Forensic pathology ; NIJ grant-related documents
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2002-IJ-CX-K001
  Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America
  Country: United States of America
  Language: English
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