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Developing Fingerprints in Blood: A Comparison of Several Chemical Techniques

NCJ Number
217118
Journal
Journal of Forensic Identification Volume: 57 Issue: 1 Dated: January/February 2007 Pages: 76-93
Author(s)
Bonnie Marchant; Christina Tague
Date Published
January 2007
Length
18 pages
Annotation
This study compared four techniques for chemically developing prints in blood: amido black, coomassie blue, ABTS [2,2'-azino-di-(3-ethylbenzthizaoline-6-sulfonic acid)], and fluorescein (9-(o-carboxyphenyl)-6-hy7droxy-3H-xanthen-3-one).
Abstract
The three top-performing procedures were amido black, coomassie blue, and ABTS. Each produced a print of a different color: black, blue, and green, respectively. This should be taken into account when the print is on a colored surface. Regarding ease of use, coomassie blue and amido black ranked first. Each can be prepared in bulk and stored over time with little loss of function. They both involve two-step procedures. The application of a working solution is followed by a rinse, each of which can be sprayed or dipped. The methanol-based formulation of amido black is more hazardous and has more potential to damage evidence than the water-based formulation. ABTS requires the additional activation step. This requires carrying more supplies into the field. This technique also involves an extra fixation step that is not needed for either amido black or coomassie blue. Fluorescein requires the use of an alternate light source, goggles, and filters for viewing and photographing developed prints. It also has a shorter shelf life than any of the other techniques. Fresh preparation is required for each use. Latent prints developed with each technique were evaluated according to the clarity of the prints produced and the level of detail observed. Each technique was also assessed for its efficiency according to preparation and development times as well as overall cost and safety. 4 tables, 1 figure, and 11 references