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NCJRS Celebrates National Library Week April 12-18

National Library Week

Started in 1958, National Library Week is a nationwide observance celebrated by all types of libraries - including the NCJRS Virtual Library. NCJRS invites you to explore the breadth and scope of the NCJRS Virtual Library collection and services. With more than 220,000 collection documents and 60,000 online resources, including all known Office of Justice Programs works, it is one of the world’s largest criminal justice special collections.

We encourage your Feedback. Tell us how you use the NCJRS Virtual Library and Abstracts Database, how you access the collection, and any ways we can improve our services.

NCJRS Abstract

The document referenced below is part of the NCJRS Library collection.
To conduct further searches of the collection, visit the NCJRS Abstracts Database.

How to Obtain Documents
NCJ Number: NCJ 223977     Find in a Library
Title: SNP-Based Microarray Technology for Use in Forensic Applications' Final Technical Report
  Document URL: PDF 
Author(s): Giulia C. Kennedy
Date Published: 2008
Page Count: 43
  Annotation: This project enabled the detection and analysis of forensically relevant single-nucleotide polymorphisms (SNPs), which will enhance and complement the genomic information currently being used for forensic DNA identification.
Abstract: Using Affymetrix 500K nuclear SNP genotyping arrays, the project demonstrated that preamplification using whole genome amplification (WGA) on low template samples yields successful genotyping for 80-90 percent of the markers on the 500K arrays. These results represent approximately 5-10 percent lower call rates than the non-preamplifier standard assay. Degraded samples prepared by DNase I digestion provide genotype data on many thousands of SNPs in the 500K assay. Using the standard assay, researchers detected mixed samples composed of two, three, or four components. In mixtures with two components, researchers detected as low as a 5-percent contribution from the minor component. The project ran the 500K SNP assay on all samples and analyzed the data using the standard algorithm. The results show that all samples, except for hair shaft (no root) yielded useable genotype data; genders were correctly assigned in all cases; mixtures were detected, and the two components of the mixture were identified; semen and buccal samples yielded high call rates comparable to control samples; vaginal samples had 5-10 percent lower call rates than semen and buccal samples; WGA samples yielded 5-10 percent lower call rates than unamplified genomic DNA; and all ethnicities were correctly identified, including a sample with ancestry from a Taiwanese aborigine. The project focused on developing an accurate, affordable micro array-based forensic DNA analysis assay capable of rapid, simultaneous, SNP genotyping for human identification testing for three forensic sample types. These were characterized by low template single-donor samples; degraded samples; and mixtures that contained two or more DNA sources. 9 tables, 14 figures, and 39 references
Main Term(s): Criminology
Index Term(s): Suspect identification ; Science and Technology ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting
Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
Grant Number: 2005-DA-BX-K101
Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America
Type: Report (Study/Research)
Country: United States of America
Language: English
  To cite this abstract, use the following link:

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