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NCJRS Abstract

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  NCJ Number: NCJ 226811   Add to Shopping cart   Find in a Library
  Title: Determination of the Age (Time Since Deposition) of a Biological Stain
  Document URL: PDF 
  Author(s): Jack Ballantyne Ph.D.
  Date Published: 08/2008
  Page Count: 84
  Annotation: This study examined changes to hemoglobin spectral profiles, changes in enzymatic activity, and degradation to RNA in order to determine the time since deposition (TSD) or the age of dried bloodstains.
  Abstract: The study observed a previously unidentified hypsochromic shift (shift to shorter wavelengths) of the Soret band of hemoglobin, which allowed for distinguishing the differing age of bloodstains by minutes, hours, days, weeks, and months. The resolution and sensitivity of this method makes it particularly suited for use with forensic casework bloodstains. In addition, the loss of activity for six enzyme candidates was used to determine the age of dried bloodstains. Although this method was limited to determining month differences in bloodstain age, it provided initial support for the use of changes in enzyme activity in determining the age of a stain. Additional enzyme candidates may be identified that could improve the preciseness of this method. Initial work focused on examining degradation to mRNA in dried bloodstains, specifically the measurement of a progressive increase in the amount of degradation, so as to determine the time since deposition; however, all RNA-based methods provided inconsistent results and were therefore unsuccessful. The descriptions of materials and methods address the preparation of body fluid stains, environmental samples, humidity studies, RNA isolation, DNase I digestion, RNA quantitation, cDNA synthesis, polymerase chain reaction, gel electrophoresis, protein extraction and quantitation, UV-visible spectroscopy, and enzyme activity assays. 23 figures, 6 tables, 47 references, and appended listings of related presentations and publications
  Main Term(s): Criminology
  Index Term(s): Biological influences ; Time of death determination ; Forensics/Forensic Sciences ; Investigative techniques ; Time of offense determination ; Blood stains ; NIJ final report
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2005-MU-BX-K071
  Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
  Type: Report (Study/Research) ; Report (Technical)
  Country: United States of America
  Language: English
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