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Validation of the Short Amplicon Multiplex Q8 Including the German DNA Database Systems

NCJ Number
227709
Journal
Journal of Forensic Sciences Volume: 54 Issue: 4 Dated: July 2009 Pages: 862-865
Author(s)
Kathrin Miller Drs.; Gunter Braunschweiger Ph.D.; Rachel Klein Ph.D.; Erich Miltner M.D.; Peter Wiegand Ph.D.
Date Published
July 2009
Length
4 pages
Annotation
Since short tandem repeat (STR) kits with shortened amplicon lengths increase the success rates of polluted stains and stains with little and degraded DNA while avoiding the problems of decreased polymorphy and the lack of an existing database, the current study developed such a STR concept that included the eight German DNA database systems (Q8) and amelogenin.
Abstract
The STR multiplex Q8 generated better typing results than the commercial kits. Compared with the MPX2 and Nonaplex kit, the average success rate was increased by using the Q8 kit by approximately 20 percent and 30 percent, respectively. Q8 is a sensitive multiplex, so weak stains can be analyzed well in combination with low copy number (LCN) PCR. In addition, the typing of highly degraded DNA samples such as bones and telogene hairs is enabled with this concept. Since the Q8 contains the eight DNA database systems, a comparison with the DNA database is possible. The first step tested and optimized the STR multiplex Q8 on different cell lines and on stains with sufficient DNA such as blood, saliva, and sperm. In the second step, researchers improved the concept for use with bones, hairs, and skin-cell mixtures. For the validation of the Q8 concept, approximately 50 biological stains with little and highly degraded DNA were examined using LCN PCR. 4 tables, 3 figures, and 18 references

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