The testing demonstrated that RSIDtm-Saliva is accurate, reproducible, and highly sensitive for human saliva. The procedure detected less than 1 ml of saliva. The sensitivity of RSIDtm-Saliva allows investigators to sample a fraction of a questioned stain while retaining the majority for DNA-STR analysis. The RSIDtm-Saliva identified saliva from a variety of materials, including cans, bottles, envelopes, and cigarette butts; and it did not cross-react with blood, semen, urine, or vaginal fluid. The descriptions of materials and methods address the sources of the materials used in RSIDtm-Saliva buffer components, the configuration of the salivary amylase lateral flow test, quantification of the salivary amylase lateral flow tests, specimens used in the testing, the preparation of body fluid extracts, the preparation of mock casework extracts, and DNA extraction and STR DNA analysis. 6 figures and 9 references