Numerous potential effects on human health are posed by the presence of such antibiotic residues in consumed food, including the risk of increased resistance of pathogenic bacteria toward the antibiotics and adverse human health effects after consumption, as well as the inhibition of starter cultures used in cheese and yogurt production. In enabling adequate risk evaluation and guiding subsequent action, sophisticated and robust analytical methods are needed to screen for a wide variety of antibiotic residues that may be present due to the choice of animal feed. The system proposed in this paper uses a simple but efficient multiresidue sample extraction procedure in order to isolate the antibiotic residues that are then identified directly with the use of high-affinity monoclonal antibodies presented in a competitive immunoassay with conjugated antibiotic hapten-chips. The hapten-chip can analyze six samples each for eight antibiotics on a single chip within 3 hours. The analytical results with both artificial positive standard samples and the incurred samples show that the antibody hapten-chip system has a comparable accuracy and a similar sensitivity to a standard ultra performance liquid chromatography-mass spectrometry assay. 2 figures, 5 tables, and 13 references