skip navigation

Add your conference to our Justice Events calendar


Register for Latest Research

Stay Informed
Register with NCJRS to receive NCJRS's biweekly e-newsletter JUSTINFO and additional periodic emails from NCJRS and the NCJRS federal sponsors that highlight the latest research published or sponsored by the Office of Justice Programs.

NCJRS Abstract

The document referenced below is part of the NCJRS Library collection. To conduct further searches of the collection, visit the NCJRS Abstracts Database. See the Obtain Documents page for direction on how to access resources online, via mail, through interlibrary loans, or in a local library.

  NCJ Number: NCJ 228279   Add to Shopping cart   Find in a Library
  Title: Development of a Multiplex PCR and Linear Array Probe Assay Targeting Informative Polymorphisms Within the Entire Mitochondrial Genome
  Document URL: PDF 
  Author(s): Cassandra D. Calloway ; Sarah M. Stuart ; Henry A. Erlich
  Date Published: 2009
  Page Count: 115
  Annotation: In order to increase the information provided by mitochondrial DNA (mtDNA) analysis by targeting additional sequence polymorphisms outside the HVI/II regions, the project described in this report developed a highly sensitive, easy-to-use, 5-plex and 10-plex PCR and linear array assay for simultaneous analysis of polymorphic regions in the noncoding and coding regions.
  Abstract: This assay targets 61 polymorphic sites distributed throughout the mitochondrial genome, using 15 primer pairs and 105 sequence-specific oligonucleotide probes immobilized in lines on a nylon membrane. The 5-plex PCR is used to amplify 5 regions that range in size from 314-444 bp; the 10-plex PCR is used to amplify 10 regions that range in size from 103-183 bp. The 5-plex probe panel consists of 59 probes and targets variation at 14 HVI sites, 11 HVII sites, 8 coding region (CR) sites, and 2 variable region I (VRI) sites. The 10-plex probe panel consists of 46 probes and targets 17 CR sites, 4 VRI sites, and five VRII sites. A population study was conducted in order to determine the power of discrimination for the new expanded HV+ array. In order to determine the assay limitations, a developmental validation study was conducted. A sensitivity study showed that both the 5-plex and 10-plex assays were highly sensitive assays, with the 10-plex being more sensitive. Additional developmental studies - including reproducibility, precision and accuracy, and environmental samples - were also completed. In addition, a procedure was validated for automating the typing assay, and scanning and interpretation software were modified for use with the 5-plex and 10-plex assays. Up to 48 samples can be typed manually or automated in less than 2 hours. The data can now be quickly analyzed with the Strip Scan Mitotyper software. 19 figures, 6 tables, and 42 references
  Main Term(s): Criminology
  Index Term(s): Victim identification ; Suspect identification ; Forensics/Forensic Sciences ; Investigative techniques ; DNA fingerprinting
  Sponsoring Agency: National Institute of Justice (NIJ)
US Department of Justice
Office of Justice Programs
United States of America
  Grant Number: 2005-MU-BX-K074
  Sale Source: National Institute of Justice/NCJRS
Box 6000
Rockville, MD 20849
United States of America

NCJRS Photocopy Services
Box 6000
Rockville, MD 20849-6000
United States of America
  Type: Report (Study/Research)
  Country: United States of America
  Language: English
  To cite this abstract, use the following link:

*A link to the full-text document is provided whenever possible. For documents not available online, a link to the publisher's website is provided. Tell us how you use the NCJRS Library and Abstracts Database - send us your feedback.