The findings indicate that COI nucleotide sequence analysis is suitable for species identification of Korean Calliphorinae flies. Furthermore, the results can be a reference for the future taxonomic revision in the subfamily Calliphorinae. The intraspecific sequence distances range from 0.0 to 0.4 percent; whereas, interspecific distances range from 3.8 percent to 6.4 percent, which indicates that species-level identification is possible with these sequences. The percent distances were calculated between the data for the current study and previously reported sequences. The phylogenetic tree generated from the current data and previously reported sequences of Calliphorinae species revealed a parphyly of the genus Calliphora. A. grahami, Eucalliphora latifrons (E. latirfrons AF295557), T, calliphoroides, and Cynomya cadaverina (C. cadaverina AF259505) occupied positions scattered among the species in the genus Calliphora. This phylogenetic result coincides with taxonomic views on the genera Aldrichina and Eucalliphora by Rognes and Whitworth, which consider genera Aldrichina and Eucalliphora as synonymies for the genus Calliphora. This suggests that the genus Triceratopyga may also be a synonymy for the genus Calliphora. 3 tables, 1 figure, and 24 references