U.S. flag

An official website of the United States government, Department of Justice.

NCJRS Virtual Library

The Virtual Library houses over 235,000 criminal justice resources, including all known OJP works.
Click here to search the NCJRS Virtual Library

Generation of DNA Profiles from Fabrics without DNA Extraction

NCJ Number
229911
Journal
Forensic Science International: Genetics Volume: 4 Issue: 2 Dated: February 2010 Pages: 137-141
Author(s)
Adrian Linacre; Vera Pekarek; Yuvaneswari Chandramoulee Swaran; Shanan S. Tobe
Date Published
February 2010
Length
5 pages
Annotation
This article is a report on a process to obtain full DNA profiles using direct amplification from a range of fabrics.
Abstract
DNA profiles can be obtained from fabrics where a person has made direct contact with clothing. A standard approach is to cut out a section of the fabric and then use a commercially available method to extract and isolate the DNA. Alternative methods to isolate DNA include the use of adhesive tape to remove traces of cellular material from the fabric prior to extraction. The absence of an extraction step both reduces the opportunity for contamination and reduces the loss of DNA during the extraction process, increasing the sensitivity of the process of generating a DNA profile. The process does not require the use of commercially available extraction kits, thus reducing the cost of generating a DNA profile from trace amounts of starting material. The results are in part dependent upon the nature of the fabric used to which the DNA has been transferred. Tables, figures, and references (Published Abstract)